35:30 – After picking up the death ray and finishing his song, Captain Hammer gives Dr. Horrible the finger.
38:39 – After Dr. Horrible’s victory, he is seen robbing a bank. In the background Moist has trouble holding on to the bags of money because his hands are moist.
38:49 – All the former Captain Hammer groupies are now groupies of Dr. Horrible. And instead of wearing Captain Hammer shirts they are now wearing shirts with Dr. Horrible on them.
39:15 – The League of Evil consists of Bad Horse, Professor Normal, Fake Thomas Jefferson, Tie-Die, Fury Leika, Snake Bite, and Dead Bowie who is played by Jed Whedon, the Whedon brother responsible for Dr. Horrible’s score and orchestration.